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Go ahead and take guesswork out of your next workflow. Get tips and methods so you're able to sequence with self-assurance.

For that i5 index, assess coloration harmony using the ahead orientation for MiSeq runs and MiniSeq operates with quick reagents, as well as the reverse-enhance orientation for all other instruments.

Seek advice from Index 2 (i5) Orientation For more info regarding how to enter i5 bases about the sample sheet in forward or reverse complement orientation.

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This permits massive quantities of libraries being pooled and sequenced concurrently throughout only one run. In comparison with other indexing methods, one of a kind dual indexing mitigates index hopping by filtering hopped reads.

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When assessing index sets for colour harmony, the sequence noticed because of the instrument needs to be assessed, whatever the orientation used for the sample sheet. For your i7 index, often asses colour stability using the "i7 bases for sample sheet" column orientation.

For operates uploaded manually (i.e., an Illumina Software is not getting used to crank out the sample sheet), the index orientation will depend on which instrument you happen to be making use of. Make sure you see the Index Orientation Desk for information on how to enter the i5 sequence depending on the instrument and program applied.